FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947679
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG28938930.536605749415152No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC10028410.58206417995967No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA761638.036793049123174No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA678437.158858643063739No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT534365.63861813968654No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG506015.339466211660278Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT356763.7645658498288976No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA290353.0638011394153506No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT226672.3918436517006287No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC217172.2915987375472078No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT203262.1448190790341455No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT197342.0823506693722242No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC110071.1614692316702175No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT91160.961929092023776No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC69870.7372749633578458No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGG65160.6875745901302024No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA58580.6181417969586749No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG56260.5936609337127867No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA53200.5613715192591584No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT48010.5066061398427105No Hit
GAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGCG40800.4305255260483772No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA38270.40382872259488706No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG37190.39243245867007714No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT34260.3615148167259167No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT33840.3570829363107128No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA29490.3111813177246726No Hit
TGAAGACAGGACAGAGCATGACACTGATCGCAACTGCAAATCAGGGCTCT27260.28765014313918535No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA26360.278153256535177No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA26300.27752013076157644No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC25410.26812876511983486No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACAGTGTCT19970.21072536164671793No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC18120.1912039836273675No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT13640.1439305925318594No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT13400.14139808943745721No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC11650.12293192104077436No Hit
GACGATCCAGTGTCAAGTCGATAGCCAAGTCACCATGATGTTCTGGTACC11380.12008285505957186No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA10500.11079701038009707No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGA10480.11058596845556354No Hit
GATCACAGAGACAGGAAGGCAGACATAGAGCACCAGGCAGACCAACCAAA10070.1062596090026264No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGCTCACAGCAACTGGACAG9980.10530992034222558No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACC9760.10298845917235688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT157.240553E-6294.994295
ATTCTGG298700.0293.46321
GATCTTG44200.0292.991765
CTTGCGC44350.0292.665958
TCTTGCG44300.0292.663337
GGAGTCA299450.0292.334176
ATCTTGC44300.0292.330386
TTGCGCT44500.0292.01099
TTCTGGA300150.0291.65242
TGATCTT44450.0291.343874
GCCACTC54600.0290.671728
TCAATGG22700.0290.445629
GAGTCAC303300.0288.623357
ATCTACC56550.0288.473335
AAGTGAT45050.0288.445831
GTGATCT45000.0288.438573
GTCACAC303950.0288.15179
AGTCACA304800.0287.15468
TGTGACC21100.0286.605531
TGACCCT21050.0285.884923
Produced by FastQC (version 0.11.5)