FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891843
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT19553821.925159473135967No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG13913915.60128856760663Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA11020412.356883442489316No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG876969.833120851988522No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC734628.237100027695456No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC289623.24743256380327No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT181702.037354108290361No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA146721.6451326074208128No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT95581.0717132948287984No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA78800.8835635868645041No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC71870.805859327258273No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA70930.795319355536793No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG68770.7710998460491365No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT61610.6908166571919049No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA56060.6285859730916764No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT54620.6124396334332388No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA47780.5357445200556601No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA43140.48371742560069425No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC33670.377532816874719No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCANGAATGCCG33040.3704687932741525Illumina Paired End PCR Primer 2 (96% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA31580.35409819889823657No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGCAATGCCG27530.30868661860888075Illumina Paired End PCR Primer 2 (96% over 31bp)
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT27500.3083502365326633No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG26200.2937736798965737No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT26060.2922038968742256No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA21850.24499827884504338No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC20860.2338976703298675No Hit
GAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGCG19450.2180877127476473No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAAGAATGCCG17080.19151352872646868Illumina Paired End PCR Primer 2 (96% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA16830.18871034475798992No Hit
CTGATGTTACCCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCC16680.18702843437690264Illumina Paired End PCR Primer 2 (100% over 30bp)
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA16170.181309939081206No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT15280.17133060415342163No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACTACACTGTCT11600.13006773613741432No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC11450.12838582575632707No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCATGAATGCCG10870.12188243894945634Illumina Paired End PCR Primer 2 (96% over 31bp)
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGTCACTGTCT10790.12098542007954315No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAACCAATTTC9550.10708162759588852No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA9430.10573609929101871No Hit
GATCACAGAGACAGGAAGGCAGATCGGAAGAGCGGTTCAGCAGGAATGCC9160.10270866060506166Illumina Paired End PCR Primer 2 (100% over 30bp)
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGAAATGCCG9000.10091462286523525Illumina Paired End PCR Primer 2 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTGG100300.0290.89211
TTCTGGA100450.0290.45772
ATCTACC167900.0288.071875
TGACATC168500.0287.570531
AACAGCC230450.0287.405154
GGAGTCA101600.0287.170076
CTACCAG168950.0285.584147
TCTACCA169000.0285.499636
AGGTCAC137300.0285.01611
TACCAGA169650.0284.40588
ACAGCCA233250.0283.891945
AAAGTCG102000.0282.722841
GTCGCAA101950.0282.717044
GTGACCC36450.0282.03711
GAGTCAC103850.0280.948277
AAACAGC235950.0280.705753
TCGCAAA102950.0278.969455
CGCAAAG103000.0278.8346
ACATCTA174000.0278.22673
CATCTAC174100.0278.15154
Produced by FastQC (version 0.11.5)