FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891843
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT13575015.22128894883965Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT33980.38100876499563263Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT18880.21169645329951575Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAGGATCGGAAGAGCGTCGTGTAGGGAAAGAGT16850.18893459947546823Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGGTAACATCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG16420.18411312304968475Illumina Single End PCR Primer 1 (100% over 30bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGACAGAGT10140.1136971417614984Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA163250.0291.29528
GGTAGAT161300.0290.612889
TCTGGTA165950.0288.954136
CTGGTAG167000.0288.108347
GGGTCTG176700.0273.043333
GGGGTCT177150.0272.765782
TGGGGTC181250.0271.600981
TTGGTAA15350.0264.08667295
TCGGTAA7350.0254.70561295
GGTCTGG209200.0230.484094
CTGGTAA10650.0225.61093295
TGGACCC168700.0210.49599295
TGGACCA13550.0208.87383295
TTGGTAC12700.0197.31828295
TGGGTAA3050.0193.322438
ATCCGGT1100.0187.610645
ATGGGGT5650.0174.802845
GGGTAAC3400.0173.42169
GGTCGAT950.0170.683369
GGTAGTT1650.0169.742959
Produced by FastQC (version 0.11.5)