FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270876
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG6517624.0611940518909No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA3060511.298527739629941No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT269259.93997253355779No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT233498.61981127896159No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC197847.303710923079194No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC196167.241689924541118No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG70202.591591724626767Illumina Paired End PCR Primer 2 (100% over 31bp)
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA68142.5155421668955538No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT38741.4301746924792156No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT35751.3197920819858533No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC27060.9989810835954458No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA24800.9155480736573192No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG18950.6995820966050886No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG18630.6877685730740265No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA18460.6814926386981497No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC17360.6408836515601235No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT14050.518687517535699No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT10570.39021544913539774No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA8720.32191851622144446No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA8630.31859596272833324No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA8610.3178576175076419No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT7690.2838937373558381No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA7560.2790944934213441No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT7490.27651028514892423No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA7270.26838848772131896No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGNAGCCCGCT7190.2654351068385534No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC6510.24033136933504629No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA6270.23147122668674963No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA6070.2240877744798358No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC5970.22039604837637886No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCGACTGGACAG5610.20710583440393387No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC5580.20599831657289683No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTACATG5540.20452162613151403No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCTACTGGACAG4540.16760436509694474No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCATCTGGACAG4390.16206677594175933No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT3940.14545400847620316No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA3860.1425006275934376No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA3780.13954724671067206No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCCACTGGACAG3780.13954724671067206No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA3640.13437883016583232No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGCAGTTAAC3200.11813523531062184No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAGCTGGACAG3190.11776606270027615No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGAAGCCCGCT3170.11702771747958475No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG2910.10742922961059674No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA2830.1044758487278312No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)