FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270876
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT69882.579778201095704Illumina Single End PCR Primer 1 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACG108.477387E-4294.814854
GGATGTC157.25242E-6294.814853
GGGTATA157.25242E-6294.814858
GGCGTAT108.477387E-4294.814857
TTATGAC157.25242E-6294.814853
GATCCGT305.456968E-12294.814859
CTAAGTT157.25242E-6294.814855
AATGGGT108.477387E-4294.814859
ATGTATA108.477387E-4294.814858
GTTACGG500.0294.814854
AGTGCGA157.25242E-6294.814859
GTTAATC255.329639E-10294.814854
GAGGTTA108.477387E-4294.814857
CAAATTG108.477387E-4294.814852
CGTACGT157.25242E-6294.814859
GGGTTTT108.477387E-4294.814858
CGTGTAT108.477387E-4294.814859
CGGCTCG206.19566E-8294.814857
TACTAGG305.456968E-12294.814858
CGATGGT108.477387E-4294.814853
Produced by FastQC (version 0.11.5)