FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464284
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC9167019.74438059463604No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG8154317.563172540944766No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC5898112.70364690577319No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA343767.404088876635852No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT317706.842794496471987No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT119852.58139414668608No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG99072.1338232633474337Illumina Paired End PCR Primer 2 (100% over 31bp)
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT97872.107977014068975No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA67681.4577284593050805No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC44790.9647112543184775No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT43050.9272341928647121No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA42570.9168956931533285No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC39970.8608954863833344No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT39580.8524954553678352No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG38540.8300953726598375No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA28820.6207407535043207No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC27820.5992022124389382No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA27560.5936021917619388No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC27230.5864944732103626No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA25520.5496635679885588No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG24960.5376019849919446No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT23760.5117557357134858No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA18880.40664765531441965No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC18340.39501684313911317No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA17030.36680135434346217No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT15280.329108907479043No Hit
GAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGCG13410.28883183568677795No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAACCAATTTC12030.25910864901655023No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA10820.23304701432743752No Hit
AGGGGACAGGATGTAACTCTCAGGTGTGATCCAATTTCGAGTCATGCAAC9760.21021616079813218No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGCAGTTAAC9710.20913923374486307No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA9690.2087084629235554No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC9160.19729303615890273No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA9050.1949237966417107No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT8770.1888930051434036No Hit
AGGGGACAGGATGTAACTCTCAGGTGTGATCCAATTTCGGGTCATGTATC8480.1826468282344427No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT7650.16476983915017532No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT7520.1619698288116756No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCGACTGGACAG7450.16046213093709885No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGACAGAAGTTAAC7240.15593903731336853No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA6880.14818516252983088No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA5920.12750816310706378No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCATCTGGACAG5730.12341584030464113No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT5720.1232004548939873No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAAAAGTTAAC5480.11803120503829552No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC5240.11286195518260375No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCTACTGGACAG5200.11200041353998846No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAATAAGTTAAC4890.10532346580971991No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCCACTGGACAG4650.10015421595402814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTT157.282537E-6294.54282295
ATTCTGG94700.0292.676671
ATTCATC14600.0292.52544
TCTTGCG5400.0291.8477
CTTGCGC5400.0291.8478
TCAATGG14550.0291.53779
TGACCCA68600.0291.53732
ATCTACC12550.0291.022375
TATTCAT14700.0290.53543
GCCACTC35000.0290.36638
ATCAATG14650.0289.547678
TTGCGCT5450.0289.16959
ATCTTGC5450.0289.138346
GATCTTG5450.0289.138345
TTCTGGA95950.0288.86382
GTGACCC69400.0288.813231
AACAGCC35400.0287.886474
AAAGTCG122100.0287.426481
GTCGCAA122250.0287.073824
TCGCAAA122050.0286.820255
Produced by FastQC (version 0.11.5)