FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464284
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT96942.0879461708781695Illumina Single End PCR Primer 1 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATC255.329639E-10294.816933
ATTCGTG206.200207E-8294.81699
TGGTAGA12550.0283.07128
GGTAGAT12400.0278.1749
TCGGTAA2400.0257.9648295
TCTGGTA14150.0257.31376
TAACTTG600.0246.58371
AAGGTTG301.5861588E-9245.680772
GTTAGAC301.5861588E-9245.680779
TATAAGG301.5861588E-9245.680774
TTGGTAA4700.0244.6353295
CAGGCTA1650.0241.21384295
GTAAGTT550.0241.213845
CTGGTAG15150.0240.329287
GCGGTAT251.8868013E-7235.853532
AGTTGGA251.8868013E-7235.853538
GGGGTAT500.0235.853532
GGTATAT251.8868013E-7235.853534
GGATTTA251.8868013E-7235.853533
CTGTTGG62200.0233.67322295
Produced by FastQC (version 0.11.5)