FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596918
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT14661724.56233519511893No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC12444420.847754632964662No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA7596912.726873707946485No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG398066.668587645204199No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC222113.720946595679809No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA114061.9108152208511051No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT105661.7700923744969996No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA88101.4759146147377027No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT69971.1721878046900913No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT54670.9158711916879706No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG52170.8739893921778201Illumina Paired End PCR Primer 2 (100% over 31bp)
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT48340.8098264753282696No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC43240.7243876043275626No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT41180.6898770015311986No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT40740.6825058048174121No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA40180.6731242817271385No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC35420.5933813354598119No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA33880.5675821469615592No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA29450.4933675982295726No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG29170.48867683668443573No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC23730.3975420409503483No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA23510.393856442593455No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT22150.3710727436599332No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA21950.3677221996991211No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA21810.3653768189265527No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA20210.3385724672400564No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA17020.28513129106510443No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAACCAATTTC16430.2752471863807089No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA15520.26000221135901413No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT15160.2539712322295525No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG11760.19701198489574784No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA10890.18243711866621548No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACTACACTGTCT8500.1423981183345116No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA8420.1410579007501868No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGTCACTGTCT7790.13050368727362888No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT7270.12179227297551758No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC7220.12095463698531457No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGA7210.12078710978727396No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG7200.12061958258923336No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC7130.11944689220294916No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT6960.11659892983625891No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA6940.1162638754401777No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC6820.1142535490636905No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGCTCTCAGGTGTGATCCAATTTCG6570.11006536911267543No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAGCCAATTTC6500.10889267872639123No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT6360.10654729795382281No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGAGCTCAGGTGTGATCCAATTTC6290.10537460756753858No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACCACACTGTCT6240.10453697157733557No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC6130.10269417239888896No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)