FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596918
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT50410.8445046053226742Illumina Single End PCR Primer 1 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA157.2566254E-6294.816477
AGCGTTA157.2566254E-6294.816477
CGGTATT305.456968E-12294.816478
GTACCAA4300.0274.24786295
TGGTAGA6200.0268.66348
GGTAGAT6550.0254.307349
TTGGTAA4050.0247.50026295
CCAATTA301.5861588E-9245.680392
TTTAAGG251.8872561E-7235.853169
GGCGTTA251.8872561E-7235.853162
TCGGTAA3150.0229.3017295
CAGGCTA1550.0228.24501295
CAAGTGT202.2872418E-5221.112377
AGGTTGT403.45608E-11221.112378
GCCTATA403.45608E-11221.112373
CGTAACG202.2872418E-5221.112379
CTTACGG202.2872418E-5221.112379
ACCCTGA3700.0215.13634295
GTAAGGT900.0212.9235
TCTGGTA8300.0211.344336
Produced by FastQC (version 0.11.5)