FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994884
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330740.8279088954773155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190300.47635926349801394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142640.35705667548794906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109050.2729741339172802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.19567526866862717No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63760.15960413368698564No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.1177255710053158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40790.10210559305351545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94200.052.9699481
GTATCAA289950.043.3082961
TATCAAC401250.031.3469052
ATCAACG411500.030.6567943
TCAACGC422100.029.8947264
CAACGCA432400.029.2297655
AACGCAG445750.028.3620876
TCGTATG3100.027.8229740
ATACATG6800.026.3880061
GCTCTAG4850.024.9022431
CGGATAT700.001334924.6413529
ACGCAGA513800.024.6074717
CGCAGAG516950.024.4508538
GCAGAGT530150.023.848579
AGAGTAC546400.023.126111
CAGAGTA546900.023.11184710
CTCGTAT3650.022.68553239
TATCCGG12700.022.54868953
AGTACTT345550.022.34217313
GAGTACT348400.022.17921312
Produced by FastQC (version 0.11.5)