FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671077
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG47940.1026315772572364RNA PCR Primer, Index 20 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.050.5226141
GTATCAA162050.045.498171
TATCAAC241600.030.4442622
ATCAACG244100.030.0312753
TCAACGC251200.029.195884
CAACGCA256000.028.6888875
TCGTATG11500.028.50187940
AACGCAG264800.027.8003186
TATGCCG12350.026.5359543
CGTATGC12550.026.38850641
CTCGTAT12050.026.34170339
ATCTCGT12400.025.87670737
TCTCGTA12550.025.56742538
CGAACTA6950.024.32274229
CCGTCTT13900.023.8268747
ACGCAGA315750.023.3034827
CGCAGAG316400.023.255618
ATACGCA6350.022.8164946
GCAGAGT328800.022.3785749
AGAGTAC332000.022.15295811
Produced by FastQC (version 0.11.5)