FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671077
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140510.3008085715564098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.046.8483731
GTATCAA147150.045.007021
TATCAAC216900.030.681752
ATCAACG220500.030.3356573
TCAACGC224350.029.7843254
CAACGCA228700.029.2630625
AACGCAG237750.028.1639676
ACGCAGA285050.023.514267
CGCAGAG286350.023.395468
GCAGAGT295350.022.6825459
AGAGTAC299200.022.36905311
CAGAGTA301800.022.19801710
GTACTTT101800.021.7572514
AGTACTT106350.020.69620113
GAGTACT107050.020.59309412
AGTACAT229450.019.60636113
GAGTACA230500.019.54698212
ATACGCA7000.019.2200896
TATCCGG9700.019.2071153
TACATGG312150.019.1753622
Produced by FastQC (version 0.11.5)