FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049899
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141150.044.9268231
GGTATCA49400.043.6048771
TATCAAC191500.033.2324452
ATCAACG196900.032.3098643
TCAACGC198600.032.085414
CAACGCA203350.031.3359385
AACGCAG208350.030.6667276
ACGCAGA246500.025.8919077
CGCAGAG249350.025.5821328
AGAGTAC252450.025.22606311
CAGAGTA257050.024.82831410
GCAGAGT259050.024.6765799
GAGTACT79200.024.0893612
GTACTTT80450.023.67218614
AGTACTT80550.023.59996413
GAGTACA200200.022.33398612
AGTACAT201400.022.14952313
TACTTTT87100.022.0238215
ATACGAC850.004121902420.2941593
ACTTTTT99050.019.64514716
Produced by FastQC (version 0.11.5)