FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005402060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230106
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219980.520034249732749No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG177250.41902023258991616Illumina PCR Primer Index 6 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146250.3457360170170677No Hit
ATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG92670.21907252442373784Illumina PCR Primer Index 6 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.20358827887528114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.20082239073914457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66080.1562135795178655No Hit
ACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG60850.14384982314863978Illumina PCR Primer Index 6 (96% over 27bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56590.133779153524758No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)