FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230106
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG479131.132666651852223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG17450.044.5086751
GTATCAA256750.034.4046671
GGTATCA121500.026.8480681
AACGCAG368350.025.7006876
ATCAACG371300.025.3942873
TATCAAC372050.025.1656682
TCAACGC380750.024.7458924
CAACGCA387950.024.464495
CGCAGAG436100.021.7385928
GCGAACT9850.021.71482328
ACGCAGA436800.021.6882157
ACAACGC3700.021.4460564
GTACTTT173500.021.43660514
GCAGAGT449150.021.2137629
AGTACTT175950.021.1577213
GAGTACT177250.021.0606912
AGAGTAC452200.021.00964711
CAGAGTA456300.020.91915310
CCGAAAT11750.020.84617847
TCGCCCC12300.019.91638438
Produced by FastQC (version 0.11.5)