FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978417
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.16129056715545811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.1262120169812532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20000.10109092269223324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA200.0073634751.74357
GGTATCA44750.051.4262851
CTCGTAT1800.040.25409739
TCGTATG1900.038.13353740
TCTCGTA2050.037.0281638
CGTATGC2200.034.49566741
ATCTCGT2150.033.70110737
TATGCCG2300.032.99752443
GTGGTAT12200.032.5229841
TGGTATC12250.032.38862
GTATCAA94650.031.3859211
CCGTCTT2850.026.6295847
TATCAAC119150.024.8151662
CCGAAAT5200.024.54623447
ATCAACG121050.024.425053
GCCGTCT3200.023.7163746
TCAACGC124700.023.6824554
CCGAACG902.1289186E-422.9971113
CAACGCA129100.022.9287555
TCGCCCC5800.022.60575138
Produced by FastQC (version 0.11.5)