FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978417
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23050.11650728840279881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA200.0073641151.7423632
GGTATCA51250.048.470941
CTACGGG851.2067176E-732.4781138
GTGGTAT15350.031.9170531
TGGTATC15400.031.5832
GTATCAA101800.030.0620541
ATACATG2300.025.5013921
TCGCCCC4950.023.70245738
CCGAAAT4850.023.47372647
TATCAAC132350.023.4309942
ATCAACG133300.023.2122543
CGTTCGG902.1281425E-422.99834824
TCAACGC136400.022.6346934
CAACGCA139400.022.2223765
AACGCAG144300.021.5394886
GCGAACT5300.020.830828
TTTAGCG2850.020.5790326
GTCCGTA1056.0249324E-419.71436555
GCCGATT1258.7499095E-519.31861341
TTGCGAT900.00573571519.16674449
Produced by FastQC (version 0.11.5)