FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4749816
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85480.17996486600744113No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCA50490.10629885452404893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG14300.046.5604321
GTATCAA192100.036.4556121
ATTCGCG400.002596093834.5028630
GGTATCA73100.032.610421
ATCAACG263550.027.7628613
TATCAAC266650.027.660032
AACGCAG269350.027.241886
TCAACGC270000.027.0613064
CAACGCA273950.026.8348225
CGCAGAG315500.023.2240058
GCGAACT10700.023.2146828
ACGCAGA316200.023.1830167
CCGAAAT13700.023.16736847
TATCGAT8250.022.99827642
ATACGCA9650.022.8791146
GTACTTT134900.022.86261414
TACATGC11600.022.6017572
TCGATTA8350.022.30970644
TCGCCCC14500.022.1291938
TAGCGTG2057.4578566E-1121.8775626
Produced by FastQC (version 0.11.5)