FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135538
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.047.2231561
GTATCAA144800.044.590311
TATCAAC199300.032.343262
ATCAACG204950.031.4332733
TCAACGC207100.031.122864
CAACGCA212200.030.4557915
AACGCAG220050.029.4634026
ACGCAGA257800.025.1146747
GAGTACT99350.024.86064312
CGCAGAG261700.024.700868
CGCTATA700.001332729724.6482582
AGAGTAC264350.024.37583411
AGTACTT101950.024.15895713
CAGAGTA271500.023.79655810
GCAGAGT272900.023.7629599
GTACTTT106100.023.14897714
TACTTTT115950.021.45021815
GAGTACA191150.020.86174812
AGTACAT193400.020.58337213
ACTTTTT123550.020.38251516
Produced by FastQC (version 0.11.5)