FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963281
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.15979184922795028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.046.77831
GTATCAA174050.043.6351431
ATCAACG241150.031.5167123
TCAACGC245650.030.980334
CAACGCA251650.030.2828035
TATCAAC251750.030.2319492
AACGCAG257750.029.5661186
TATGCCG4150.028.26399443
TCGTATG4200.027.10783240
CTCGTAT4100.026.0853739
ACGCAGA298150.025.5367017
CGCAGAG299200.025.435238
CGTATGC4750.024.69380641
GCAGAGT309650.024.5879929
AGAGTAC308900.024.5806811
GTACTTT135000.024.40432714
CAGAGTA311650.024.38592110
AGTACTT138400.023.75494813
GCGAACT9500.023.60467128
GAGTACT140150.023.53187812
Produced by FastQC (version 0.11.5)