FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963281
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54000.13625074780213667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.046.148611
GTATCAA159200.043.405241
ATCAACG225900.030.9099663
TCAACGC229150.030.4561354
TATCAAC234800.029.8264942
CAACGCA234700.029.8094255
AACGCAG238950.029.3229146
GTACTTT98950.025.45137814
ACGCAGA278650.025.144577
CGCAGAG280350.024.9797888
AGAGTAC286500.024.45623411
GCAGAGT289850.024.2446829
GAGTACT104200.024.16903912
CAGAGTA291350.024.13169910
AGTACTT104950.023.96344813
GCGAACT8750.023.26235628
TAACGGC7400.022.84813736
TACTTTT110900.022.77137415
ACTTTTT112300.022.4564916
GAGTACA211150.021.3217912
Produced by FastQC (version 0.11.5)