FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881869
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.15474504678030093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58850.047.720161
GTATCAA165100.042.650081
TCGTATG3100.034.5026140
ATCAACG231300.030.321213
TCAACGC235750.029.733474
CAACGCA240850.029.1747255
TATCAAC240800.029.1551382
AACGCAG248550.028.2844166
CTCGTAT3600.027.79269239
ACGCAGA292000.024.0874717
CGCAGAG293050.024.0129368
CGTATGC4500.023.76601641
GTACTTT128550.023.7224414
TATGCCG4250.023.53901743
AGAGTAC301250.023.30295411
AGTACTT131550.023.28634513
GAGTACT132350.023.22348412
GCAGAGT304750.023.1363059
CAGAGTA306150.022.99670210
TACTTTT143450.021.4756815
Produced by FastQC (version 0.11.5)