FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881869
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59240.1526069014693695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57450.042.8925251
GTATCAA160550.042.6056061
ATCAACG226350.030.7419193
TCAACGC230550.030.211814
TAGACCG802.764551E-630.1865585
TATCAAC233450.029.8532072
CAACGCA235850.029.562155
AACGCAG239650.029.136216
GTACTTT91100.025.6365214
ACGCAGA279300.024.999657
CGCAGAG279200.024.9338258
GCGAACT8600.024.47079828
AGAGTAC288650.024.27289411
GCAGAGT290450.024.0512059
CCGAAAT10650.023.97108347
CAGAGTA293000.023.87720710
AGTACTT99100.023.60147913
GAGTACT99550.023.59875312
TCGATTA4700.023.4877244
TATCGAT4700.023.4874242
Produced by FastQC (version 0.11.5)