FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305633
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG489511.1369060019746227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG17750.041.792921
GTATCAA264600.034.464341
GCGAACT10000.026.21897128
GGTATCA124800.025.5458261
AACGCAG379900.025.3904236
ATCAACG385400.024.9567583
TATCAAC387300.024.780882
TCAACGC392750.024.437014
CAACGCA402450.024.0791915
CGAACTA11100.023.62069529
ACAACGC4200.022.177964
TACATGC12900.021.9296282
CGCAGAG449450.021.4990188
ACGCAGA450900.021.3997767
GCAGAGT461400.021.0104739
AGAGTAC466000.020.96594611
GTACTTT178200.020.90875814
CAGAGTA469850.020.86757310
TAAGACT12500.020.6994299
AGTACTT182650.020.43712213
Produced by FastQC (version 0.11.5)