FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731126
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106050.3883013819208634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG11250.042.0140041
GTATCAA150300.041.777111
GGTATCA52950.039.1592641
TATCAAC220700.029.544562
ATCAACG224150.029.1052153
TCAACGC228750.028.4445234
AACGCAG231750.028.1799966
CAACGCA232700.028.0353055
GCGAACT6600.025.6121728
ACGCAGA270500.024.1422397
CGCAGAG271150.024.058928
GCAGAGT277550.023.5782939
AGAGTAC280750.023.38327211
GTACTTT106950.023.35435714
CAGAGTA282550.023.27093510
TACATGC7900.023.1455152
AGTACTT109650.022.77928413
GAGTACT112100.022.3429812
CGAACTA7900.021.83499129
CGTGCGA953.0686852E-421.78782710
Produced by FastQC (version 0.11.5)