FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292448
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89000.20734089265612538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51940.12100321308493428No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT47870.11152144417358113No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG46820.1090752875748291No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG46250.10774737399264943TruSeq Adapter, Index 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA181650.042.6756821
GGTATCA60200.042.579951
TCGTATG8450.040.82971240
CGTATGC8950.038.5442341
TATGCCG9050.038.49951643
ATCTCGT9150.037.32993737
CTCGTAT9350.036.53058239
CGTATCT9750.035.3890534
CCGTCTT9700.034.85597647
ACGTATC9800.034.8551933
TCTCGTA10100.033.81870338
GCCGTCT10550.032.6986646
GTATCTC11200.030.8074335
GACGTAT11450.030.13126832
ATCAACG258000.029.9777033
TCAACGC263950.029.3146694
TATCAAC264900.029.1851922
CAACGCA269650.028.7330445
CGACGTA12250.028.16318531
AACGCAG275350.028.1507726