FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292448
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95710.2229729981586265No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG46580.10851616606654291No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT45690.10644275713998165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168200.041.135641
GGTATCA56450.038.7574421
ATCAACG236500.030.004493
TCAACGC241700.029.2162364
TATCAAC242250.029.1933082
CAACGCA246350.028.8328065
AACGCAG249050.028.5762966
CCGAAAT15300.025.25486847
CGCAGAG289000.024.6143918
ACGCAGA290150.024.564397
TACATGC13950.024.2351152
GCGAACT13600.024.0983928
TCGCCCC15900.024.08711638
GCAGAGT298150.024.0094159
ATACATG9100.023.5114861
CAGAGTA303900.023.47567610
AGAGTAC303650.023.47310311
TTCCGCG902.1280264E-422.99943425
AAAGCGA15200.022.46984125
GTACTTT107600.021.89913414
Produced by FastQC (version 0.11.5)