FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4801821
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88800.18492984224109976No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCA50110.10435624318357556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG15150.046.2434731
GTATCAA197250.036.182671
GGTATCA75950.034.2163961
ATCAACG277500.027.1893653
TATCAAC280250.026.8531782
TCAACGC283100.026.6396224
AACGCAG285550.026.5439596
CAACGCA289050.026.2225485
GCTCTAG4300.025.6831671
TAGCGTG1251.266435E-724.83966626
TACATGC12750.024.3566722
GCGAACT12500.024.01142928
ACGCAGA334700.022.6556327
CGCAGAG335450.022.6152618
TCTATCG2600.022.55738831
TAAGACT13700.022.4114079
AAAGCGA13400.022.39848525
CCGAAAT12200.021.49151447
GTACTTT143150.021.40148514
GCAGAGT356350.021.3370579
Produced by FastQC (version 0.11.5)