FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3884222
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318090.8189284752519295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183350.4720379010262544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137640.35435667683258065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105120.27063334690962565No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74140.1908747749227516No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60610.15604154448432658No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45050.1159820422210677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91300.050.4836581
GTATCAA272100.043.058041
TATCAAC377950.031.0347672
ATCAACG386500.030.348623
TCAACGC395150.029.6751634
CAACGCA404550.028.985275
AACGCAG414350.028.2997236
GCGAACT7100.027.69550528
TCGTATG2400.025.87476240
CGACGGT851.4446308E-424.351787
ACGCAGA483700.024.2565427
CGCAGAG484950.024.1869058
TAAGACT7850.023.731359
CGAACTA7900.023.58145729
GCAGAGT499950.023.4750259
CTCGTAT2500.023.46008939
ATACATG4950.022.999791
TACATGC8100.022.9991972
ATACGCA6200.022.8134276
AGAGTAC517600.022.681211
Produced by FastQC (version 0.11.5)