FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799203
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292100.7688454657463684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173810.4574906894946124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128610.33851836819459236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98520.25931754633800824No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68220.17956397697095944No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.14574109359252455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.11202349545417814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39790.1047324925780486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85900.051.728351
GTATCAA266700.043.747831
TCGTATG2750.042.65549540
ATCTCGT3200.036.65706637
TATGCCG3700.032.63243
CTCGTAT3800.031.77702539
TCTCGTA3700.031.70340738
TATCAAC370750.031.4688742
ATCAACG382050.030.5463373
TCAACGC390650.029.8827044
CGTATGC4050.029.81234241
CAACGCA399200.029.3200685
AACGCAG410200.028.5422236
CCGTCTT4650.025.96763847
GCTCTAG4000.025.8742161
ACGCAGA474900.024.6536547
ACATACG700.001335262424.6401638
CGCAGAG476650.024.5703758
GCAGAGT489700.023.9226449
AGAGTAC504950.023.18088211
Produced by FastQC (version 0.11.5)