FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799203
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50040.13171183535073014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG17500.054.823471
GTATCAA218850.042.9398881
GGTATCA71050.041.1414071
ACATGGA15700.031.8607943
TATCAAC322200.031.4271622
ATCAACG331150.030.5024413
TCAACGC338250.029.7601974
CAACGCA346750.029.2594955
AACGCAG351800.028.869286
TCGATAG500.007736821727.6002222
CGCTAAT906.912587E-626.83284448
GACGTGT5800.025.57678269
GTACTTT143950.025.54765114
TGCTACG1501.0440999E-925.301219
TATCGGC1501.0440999E-925.30020115
ACGCAGA412050.024.664437
CGCAGAG413200.024.570748
GCAGAGT418400.024.2818589
TACTTTT154800.023.89166515
AGAGTAC432700.023.79062811
Produced by FastQC (version 0.11.5)