FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4203409
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.045.3166921
GTATCAA146850.042.9441951
ATCAACG198850.031.8351863
TCAACGC203550.031.1351174
TATCAAC204950.030.9473652
CAACGCA209150.030.3839475
AACGCAG213900.029.7740926
CGAACTA10300.027.46625129
GCGAACT10700.027.08369628
CGCAGAG249750.025.457568
ACGCAGA254150.025.0575457
AGAGTAC256500.024.77270111
GCAGAGT262150.024.3838239
GAGTACT96450.024.10734712
CAGAGTA264550.024.01917810
CCGAAAT9600.023.71866847
AGTACTT98650.023.57000713
AAAGCGA12350.023.18477425
GTACTTT105150.022.08018514
TACATGC13650.021.488812
Produced by FastQC (version 0.11.5)