FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4288290
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.044.4807321
GTATCAA140600.042.623851
ATCAACG196000.030.8386923
TATCAAC198300.030.446212
TCAACGC199500.030.2976634
CAACGCA203450.029.760655
AACGCAG207000.029.2335936
CGCAGAG247000.024.4988388
ACGCAGA252550.024.0014387
AGAGTAC255700.023.66445511
CGAACTA10250.023.5600929
CAGAGTA258400.023.47059210
GCAGAGT259300.023.4287689
TATCGAT8700.022.20580142
AGTACTT92100.021.9510813
GAGTACT92700.021.88343612
TCGATTA8700.021.80977644
GCGAACT11100.021.7561928
CGATTAC8750.021.6851545
AGTACAT191250.021.06974613
Produced by FastQC (version 0.11.5)