FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379805
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85880.19608178902942025No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG48310.11030171434573001No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT46410.10596362166808797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64100.040.579481
GTATCAA178050.039.9714971
ATCAACG243050.029.6791573
TCAACGC247850.029.0904544
TATCAAC249350.028.9846342
CAACGCA253000.028.5664755
AACGCAG255150.028.2852026
CGGTACG1901.8189894E-1225.42475935
CCGAAAT13800.025.2493647
GCGAACT15400.025.08969528
ATACATG9250.024.9876981
CGCAGAG295700.024.3944428
ACGCAGA296800.024.3267237
TACATGC16000.024.1485582
GCAGAGT305600.023.7063149
CGAACTA16500.023.41785429
TCGCCCC14900.023.38771438
CAGAGTA311850.023.1758910
AGAGTAC310850.023.12863211
GTACTTT121150.023.00916914
Produced by FastQC (version 0.11.5)