FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156625
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185520.4463236399723333No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG161180.3877665173067092Illumina PCR Primer Index 6 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120320.28946561212522176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70420.16941629326677293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68720.16532643671247707No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52590.12652091540612878No Hit
ATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG46480.11182148979039484Illumina PCR Primer Index 6 (96% over 27bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.10929540191862389No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30200.043.98259740
TATGCCG30800.043.2341743
CTCGTAT30500.042.98491739
CGTATGC31300.042.54455641
ATCTCGT31450.041.57678237
TCTCGTA31700.041.1395638
GTATCAA268300.040.017141
CCGTCTT33150.039.33914647
TATCTCG34700.037.7848636
CGTCTTC36000.036.22566648
GACTATC37200.035.43020233
GCCGTCT37200.034.9609946
GGTATCA107200.034.404091
GTATGCC39550.033.66868642
ATGCCGT41300.032.158944
TGCCGTC41800.031.77422745
AGACTAT43600.030.78301232
CTTGAAA43300.030.359257
GCGAACT11150.030.01328528
ATCAACG391650.027.5615233