FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156625
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297720.7162541725558597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG16200.041.099661
GTATCAA250500.034.580791
GGTATCA117500.025.866281
AACGCAG359700.025.4441246
ATCAACG364450.025.0935733
TATCAAC366950.024.9226112
GCGAACT9950.024.6179628
TCAACGC372400.024.5115574
CAACGCA379300.024.2293685
TCGTTTA5350.023.21818432
GTACTTT166600.022.3239514
CGTTTAT5450.022.15904633
AGTACTT168900.022.10139513
CCGAAAT12350.022.06870847
GAGTACT170550.021.86681712
CGCAGAG422950.021.6056828
ACGCAGA424000.021.5526967
CGAACTA11550.021.50689929
GCAGAGT434650.021.1119049
AGAGTAC436600.021.05023211
Produced by FastQC (version 0.11.5)