FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4715111
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61430.13028325314080624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.041.667981
GTATCAA180000.040.1211661
CGTATGC12100.031.93135641
TATGCCG12350.031.28696343
TCGTATG12300.030.85481640
ATCTCGT12400.029.77190837
ATCAACG241800.029.7384453
CTCGTAT12650.029.1835339
TCAACGC246950.029.1185764
TATCAAC249650.028.8448122
CAACGCA252450.028.4702155
AACGCAG259000.027.736896
AATCGCG500.007733093627.60308636
CCGTCTT14200.025.99580247
AATCTCG15100.025.1352636
TCGAATC14950.024.92348533
GCCGTCT14950.024.69166446
CGAATCT15450.023.67279834
CGCAGAG304500.023.6107068
ACGCAGA307850.023.342577
Produced by FastQC (version 0.11.5)