FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339970
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235470.5425613541107427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147590.3400714751484457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105600.24331965428332453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70470.16237439429304812No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47840.11023117671320308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74550.053.3106461
GTATCAA239650.043.201381
TCGTATG3400.035.51511440
TATCAAC331250.031.2011052
ATCAACG338600.030.5540353
TCAACGC345750.029.9022354
CAACGCA355650.029.1277265
AACGCAG367400.028.1961776
CTCGTAT4300.028.08171839
TATGCCG4700.026.423443
ATCTCGT4600.025.50088137
CGCAGAG428050.024.2091488
GCGAACT5850.024.17920928
ACGCAGA428600.024.1780827
CGTATGC5150.024.11511641
GCAGAGT439250.023.5683029
AGAGTAC456750.022.66634811
CAGAGTA457800.022.61331710
CAATACG9350.022.5059474
ATACGCA9450.022.2675326
Produced by FastQC (version 0.11.5)