FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339970
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.043.022431
ATACATG14700.042.4908681
GTATCAA206300.040.263471
TATCAAC290800.030.5727332
ATCAACG294050.030.1036853
TCAACGC299000.029.6053144
CAACGCA305550.029.1851845
AACGCAG311800.028.5891086
GTACTTT110950.025.68336714
ATACGCA8500.024.7568346
CGCAGAG364300.024.4598888
ACGCAGA365050.024.4096367
GCAGAGT368800.024.1988539
GCGAACT5300.024.08408228
CCGAAAT8400.023.82088947
TACTTTT121200.023.74010815
ACTTTTT123750.023.27933316
AGAGTAC388750.023.19713811
CAGAGTA392950.023.06279810
CGTCACG750.001991235423.00530835
Produced by FastQC (version 0.11.5)