FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096488
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.10313712624081897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.046.949671
GTATCAA145950.042.8086741
ATCAACG204050.030.549343
TATCAAC207400.030.0233632
TCAACGC207550.030.0171874
CAACGCA213250.029.3277325
AACGCAG218650.028.6507556
TCGTATG2450.028.16556440
CGTATGC2700.026.8315941
CGCAGAG259100.024.1512478
TATGCCG3000.024.14754943
GCGAACT10700.023.85826528
ACGCAGA263350.023.8138857
CGAACTA10750.023.74729729
AGAGTAC267550.023.45323411
GCAGAGT272250.023.0100569
CAGAGTA273450.022.94692210
GAGTACT119650.021.76753412
TAAGCGC8350.021.48416147
AGTACTT121900.021.30941613
Produced by FastQC (version 0.11.5)