FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096488
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50760.1239110184138218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.043.6776281
GTATCAA134600.043.6149641
ATCAACG183600.032.0934563
TCAACGC186800.031.5436734
TATCAAC188700.031.2294942
CAACGCA191300.030.8918325
AACGCAG194750.030.3803836
CGCAGAG233950.025.3037518
ACGCAGA237750.024.8845027
AGAGTAC239250.024.7726511
GCAGAGT244600.024.3292439
CAGAGTA244900.024.2149210
GAGTACT85450.024.062512
AGTACTT88000.023.28682913
CCGAAAT10250.023.2237247
GTACTTT92950.022.04670114
CGAACTA9400.022.02037429
AGTACAT180750.021.35789113
TCGCCCC11150.021.3522938
GAGTACA181800.021.2724912
Produced by FastQC (version 0.11.5)