EGAF00005402126 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005402126
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4085142
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18928	0.46333762694173175	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	17611	0.4310988455236073	Illumina PCR Primer Index 6 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12412	0.30383276762472394	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7406	0.18129112770131367	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7135	0.17465733137305875	No Hit
ATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	6137	0.15022733603874724	Illumina PCR Primer Index 6 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5460	0.13365508469473034	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4700	0.11505108023172755	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	3240	40.88822	40
CTCGTAT	3270	40.514587	39
ATCTCGT	3250	40.236084	37
GTATCAA	26370	40.17542	1
TATGCCG	3315	40.16539	43
CGTATGC	3385	39.539566	41
TCTCGTA	3305	39.461136	38
CCGTCTT	3465	37.834415	47
GGTATCA	10400	37.25093	1
TATCTCG	3650	35.83016	36
CGTCTTC	3650	35.728184	48
GACTATC	3955	33.502033	33
GCCGTCT	4140	31.828138	46
GTATGCC	4315	31.337482	42
ATGCCGT	4450	29.920958	44
TGCCGTC	4550	29.187544	45
AGACTAT	4585	28.89587	32
CTTGAAA	4635	28.140976	57
TATCAAC	38540	27.602995	2
ATCAACG	38785	27.45531	3