FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085142
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335750.8218808550596283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG16500.045.5944751
GTATCAA240250.033.3820041
AACGCAG345300.025.355686
ACAACGC4000.025.0102584
GGTATCA115650.025.0056461
ATCAACG352100.024.6308523
TATCAAC352600.024.528042
TCAACGC358450.024.213764
CAACGCA367700.023.8860865
CCGAAAT12600.023.54781547
GCGAACT9050.022.48997328
TATATCG4300.021.660855
AACAACG4950.021.6041243
GTACTTT146300.021.48203814
ACATGGA20750.021.446273
CGCAGAG409450.021.4086518
ACGCAGA410250.021.3584967
TAACGGC7650.021.20144536
AGAGTAC423300.020.98629211
AGTACTT150050.020.96841413
Produced by FastQC (version 0.11.5)