FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657228
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102570.38600376030961586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG10000.043.4851461
GTATCAA147200.041.334711
GGTATCA53400.040.328651
TATCAAC216300.029.4160842
ATCAACG217400.029.307693
TCAACGC222300.028.6151264
CAACGCA227050.028.1684155
AACGCAG227300.028.152616
TATACGT500.00773280227.60286137
GCGAACT6250.025.94327228
GTACTTT106150.024.40999414
ACGCAGA265700.024.1098527
AGTACTT107600.024.08104913
CGACGTT5900.023.97390467
CGCAGAG267350.023.9098918
GAGTACT109500.023.69471412
GTTACGA2053.6379788E-1223.56164419
CCGAAAT7650.023.45120647
GCAGAGT273600.023.4141399
TCGATTA3550.023.32372944
Produced by FastQC (version 0.11.5)