FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4426306
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233080.5265790480820802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143450.3240851400693942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103520.23387447682107834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71910.1624605257747657No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46820.10577669054059977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79850.050.6382371
GTATCAA250750.042.8178181
TCGTATG2700.034.50093540
TATCAAC337200.031.768022
ATCAACG345500.031.1649743
TCAACGC352250.030.547154
CAACGCA361900.029.761215
AACGCAG372250.028.9334096
CTCGTAT3300.028.22771839
ACGCAGA434750.024.7818537
ATACGCA8500.024.7578146
CGCAGAG436000.024.7187188
GCAGAGT447100.024.1436149
TATGCCG3750.023.91929443
AGAGTAC467250.023.08027811
CAGAGTA468900.022.99092710
CAATACG9300.022.6283654
GTACTTT276100.022.45370314
TCGCCCC7850.022.41411238
AGTACTT285350.021.78628513
Produced by FastQC (version 0.11.5)