FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4514876
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104260.23092550050101043No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCA53390.11825352457077447No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGT49080.10870730447525025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG13950.047.7483751
GGTATCA66700.041.290731
GTATCAA192200.039.3606871
TATCAAC269650.029.4189662
ATCAACG271900.029.169063
TCAACGC278100.028.4697724
CAACGCA285200.028.0755255
AACGCAG288300.027.7856046
CGCAGAG339350.023.6148078
ACGCAGA339500.023.6043727
GCAGAGT347200.023.1295459
GTACTTT140650.023.08061414
GCGAACT8100.022.57370228
CAGAGTA361500.022.26280410
TCGCCCC9500.022.15643738
AGAGTAC362650.022.14464611
CCGAAAT9350.022.13916247
ACAACGC4250.021.91684
AACAACG3950.021.8341143
TACATGC8900.021.7113532
Produced by FastQC (version 0.11.5)