FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504857
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129900.28835543503378686No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG49030.10883808298465412No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT48200.10699562716419189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA187400.040.1356771
GGTATCA67050.039.7763021
ATCAACG262150.029.0842043
TCAACGC267450.028.5078474
TATCAAC270550.028.1429462
CAACGCA272250.028.1066065
AACGCAG275100.027.8282786
GCGAACT15000.027.1397328
CGTTCGG2300.026.99913224
TACATGC15950.025.9558532
ATACATG7450.025.4712411
AAAGCGA17000.024.14868725
CGCAGAG318800.024.0017838
ACGCAGA320850.023.8591827
CGAACTA17200.023.66836729
GCAGAGT330100.023.2739549
CAGAGTA337400.022.79084610
AGAGTAC337900.022.72649411
TATCCGG9150.022.6237353
GTACTTT126600.022.61926314
Produced by FastQC (version 0.11.5)