EGAF00005402144 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005402144
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4368396
Sequences flagged as poor quality	0
Sequence length	75
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT	10483	0.2399736653911413	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	9025	0.20659757036678908	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	8510	0.19480834612979225	Illumina PCR Primer Index 2 (96% over 29bp)
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCT	7518	0.17209978216260613	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG	6492	0.14861290047880274	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAACCAAAGCTAAGACCCCCGAAACC	5943	0.13604535852518865	No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG	5824	0.1333212465170282	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC	5766	0.13199352805926937	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCT	5654	0.12942965793394187	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGA	5529	0.12656819574049605	No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGT	5314	0.12164648076776922	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGAT	5115	0.11709103295580346	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGT	5099	0.11672476579504239	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG	5043	0.11544283073237865	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT	4975	0.11388619529914414	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA	4813	0.11017774029643831	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT	4664	0.1067668773618509	No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTA	4534	0.10379095668066722	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTA	4421	0.1012041948577922	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTT	4412	0.1009981695798641	No Hit

Adapter Content

Adapter graph

Kmer Content

$Kmer graph$

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	19205	43.43834	1
GGTATCA	6370	43.112663	1
TCGTATG	1765	40.0724	40
CTCGTAT	1735	39.57171	39
ATCTCGT	1755	39.318233	37
TATGCCG	1845	38.51787	43
TCTCGTA	1785	38.464138	38
CGTATGC	1865	38.104813	41
CCGTCTT	1935	36.01527	47
GCCGTCT	1960	35.905876	46
CGTCTTC	1970	35.37581	48
GTATCTC	2025	33.90659	35
ATCAACG	27865	30.146114	3
ATGCCGT	2370	29.985432	44
TCAACGC	28530	29.443445	4
TGCCGTC	2465	29.38961	45
TGTATCT	2350	29.3642	34
CAACGCA	29105	28.908506	5
AACGCAG	29960	28.11806	6
CATGTAT	2570	28.056238	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content