FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484583
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100580.22427949265294006No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCA54600.12175045037632261No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGT49740.1109133223757928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG11650.042.0492741
GTATCAA195200.039.7294541
GGTATCA68650.038.5434761
TATCAAC269050.030.028962
ATCAACG273800.029.6336693
TCAACGC280650.028.7874644
CAACGCA285950.028.3986635
AACGCAG287000.028.2833796
GTACTTT136250.025.57238614
GCGAACT8100.025.55619828
CGCAGAG333650.024.3073838
ACGCAGA334200.024.298897
GCAGAGT340050.023.8400219
ACTTTTT148400.023.6440516
TACTTTT149450.023.4312415
AGTACTT149200.023.42190413
TACATGC9350.023.2442382
GCGTATC2400.023.00006547
GAGTACT153050.022.96821412
AGAGTAC354400.022.8749711
Produced by FastQC (version 0.11.5)