FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455276
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT108480.24348659881004006No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG93270.2093472996959111No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG83400.18719379001435602Illumina PCR Primer Index 2 (96% over 29bp)
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCT76010.1706067143763933No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG65870.14784718163364066No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAACCAAAGCTAAGACCCCCGAAACC60880.13664697765076733No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC59670.13393109652465976No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG59280.1330557298807077No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCT57300.12861156076525898No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGA55480.1245265164268162No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGT55110.12369604037998992No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGAT51780.1162217559585534No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG50570.11350587483244584No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGT50200.11267539878561955No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT50140.11254072699424233No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA49790.11175514154454179No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT48470.10879236213424262No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTA46180.10365238876334484No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTT46160.10360749816621911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194000.042.840881
TCGTATG15350.042.02783640
GGTATCA66550.041.5766641
CGTATGC15450.041.53158641
CTCGTAT15350.040.9045639
ATCTCGT15450.040.86447537
TATGCCG15800.040.6111343
TCTCGTA16050.039.12138
CCGTCTT17200.036.9069247
GTATCTC17400.036.08736835
GCCGTCT18250.034.9705946
CGTCTTC18500.034.87331448
ATGCCGT20800.031.18054844
TGTATCT21100.030.24978634
TGCCGTC21700.030.20529445
CATGTAT21600.030.0280732
ATCAACG280200.029.6737373
TCAACGC286850.028.9734634
CAACGCA293800.028.404875
AACGCAG299800.027.8594076
Produced by FastQC (version 0.11.5)